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Gradient HPLC is the ideal technique for analyzing complex samples, while isocratic HPLC is often used for simple mixtures. During gradient HPLC, the strength of the solvent that carries the sample is increased, while in isocratic HPLC, it remains the same. The advantages of gradient HPLC are more specific results in the sample identification and analysis. However, gradient HPLC requires more complexes machines that can automatically increase the solvent gradients. Such HPLC machines contain a piece of equipment called a binary pump and can also perform isocratic HPLC. The BLC-20G from Buck Scientific is the binary gradient version of our BLC-20 analytical HPLC systems with variable wavelength detectors.

The BLC-20G HPLC system uses a high-quality,
precision monochromator based spectrophotometer for a full
range of wavelengths from 190-800nm for selective analyses
integrated into a complete LC package.
The 20-series is easily upgraded to a gradient System with the
Buck-Chrom controller & Peak-Simple data transfer, optional
semi-prep systems for material isolation with optional Auto-
Sampler, Fraction Collector, RI Detector, & Fluorescence detector.

Pump Spec.s:
Flow Rate 0.01-9.99 ml/min.
Pressure 0-2500PSI (Analytical)
Accuracy 1% Max. deviation
Precision <0.5% RSD
Panel Controls Run/Stop & Prime, Flow
Control, Pump Status LED
Display Flow rate (ml/min)
Shipping Weight 40 lbs.
Dimensions 16"W x 7"H x 17"D

Detector Spec.s:
Wavelength Range 195 - 800nm
Bandpass 5nm
Flowcell 10L Volume, 7mm Path
ABS Range 0.005 - 1.0 ABS (2 ABS Max.)
Drift <2 x 10-4 A/hour @ 254nm
Noise <2 x 10-5 A @ 254nm
Linearity >2%
Panel Controls ABS, Auto-Zero,Wavelength
Wavelength Accuracy <2nm, Reproducibility < 1nm
Recorder Output 10mV full-scale
Integrator Output 1V/ABS to 2V
Display 4 Digits (ABS), 3 Digits (NM)

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