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Integral Control System

All control software is within the instrument, tamper proof, providing easy menu driven operation with massive safe storage for scans, plots, curves etc. PC control and data transfer to a PC are provided.

Equal Sample and Reference Facilities

Identical and generous sample and reference sampling facilities widens the scope of measurement, for example, to enable multi-cell sampling in both beams and accommodate bulky accessories in the reference beam.

Method Operation and Storage

Up to 30 sets of instrument parameters, including concentration curves may be stored, security code protected. When recalled, instrument parameters are automatically set, avoiding errors.

Alpha Numeric Entry

Full Alpha Numeric entry is provided so that results may be fully documented for GLP. Formula entry enables the creation of a wide range of formulae for automatic calculations using many wavelengths.

Software Enhancement By E-SEF

E-SEF - Encoded Software Enhancement Facility - allows software modules to be enabled using encoded numbers provided by telephone, fax or E-mail. Software requirements may be tailored, at any time, as required.

Data Storage and Protection

Data, including 100 spectra, concentration curves , kinetics and time course plots may be stored in safe memory, security code protected against tamper or deletion using a four digit password.

Reprocessing

Scans, time course plots, and reaction curves may all be reprocessed to any required format on the display screen and then plotted on the integral or an external printer.

Performance Validation

Software and certified standards enable wavelength accuracy, Absorbance accuracy, bandwidth etc. to be validated. A timed and dated report includes the serial number of the instrument.
Quantification

Powerful Quantification

Many quantitative methods are available including band area computation with background correction, differencing and stripping of spectra, wine and colour assays, mathematical calculations between several wavelengths, protein and DNA assays.

Curve and Line Fitting

A linear, quadratic or cubic concentration curve may be fitted to up to 30 standards, with or without an intercept at the origin. Standards may be deleted and replaced and a new curve fitted.

Reaction Kinetics

Single cell kinetic measurements are possible with plots and data displayed on screen. Data may be reprocessed, regression analysis applied and curves stored, security code protected against tamper or deletion.

Multi-Cell Reaction Kinetics

Up to four or six cells are automatically measured with all reaction curves displayed on screen. Each curve may be selected for processing with the results of regression analysis printed.

Multi Wavelength Assays

Assays are provided using either two or three wavelengths. A wine colour assay is provided which may use up to ten different wavelengths. Computations at up to four wavelengths are also provided.

DNA and Protein Assays

Protein assays may be made using either the 2 or 3 wavelength methods of Warburg and Christian. Also programmed are techniques and analytical procedures for assays by Lowry, Bradford, Biuret and BCA methods.

Band Area and Height Computation

Band areas with correction for sloping background absorption are readily quantified, with all details and construction displayed. Band areas may also be computed for derivative spectra to facilitate difficult quantification.

Wavelength Program

Using the various cell changers, measurements may be made on up to 4, 6 or 8 cells at a single wavelength with cycle time and number of cycles entered by the operator. A cell and wavelength program may be combined.

Cell Program

Measurements may be programmed for 10 different operator selected wavelengths with the cycle time and number of cycles selected by the operator. A wavelength and cell program may be combined.
Scanning

Double Beam Wavelength Scans

cans at up to 4000nm/min are presented on screen or printed with all peaks annotated with wavelength and absorbance. Tables are also presented listing all peaks and troughs with values.

Display Scrolling

Use of the display scrolling facility enhances the effective screen size and enables spectra and data up to 550nm wide to be viewed.

Dynamic Scan Recall

The last eight scans are automatically retained for recall by entering their scan sequence number and may be reprocessed, overlayed or transferred to protected memory for future recall.

Overlayed Spectral Scans

Up to eight scans, including stored scans, may be overlayed, with or without offsets for convenient comparisons or further manipulation.

Difference Spectra

New wavelength scans or scans from store may be presented on screen and the difference spectra produced. The scans may be normalised enabling spectral stripping to reveal impurities or additives.

Derivative Spectra

Derivative spectra, up to fourth are readily produced, with selectable smoothing, either alone or superimposed on the fundamental, with or without an offset. Scaling is automatic with all peaks and troughs quantified.

Scan Storage

Up to 100 spectra may be stored in 10 safe memory banks for future recall. Each spectrum is automatically allocated a recall number, when stored, and may be security code protected against tamper or deletion.

Programmed Scans

Automatic scans, single or repeat may be made of a cell program of up to 4, 6 or 8 cells. A programmed sequence of scans may also be made of a single sample.

Time Course Plots

Changing samples or flows may be examined and displayed by making a time course plot. Data may be reprocessed and stored, security code protected, with an automatically assigned recall number.
Sampling

Sampling Flexibility

The design of the double beam optical sampling allows exactly similar space and facilities in both the sample and reference beams.  Full size accessories and multi-cell reference sampling, with temperature control is therefore possible.  Operation is not limited to a single standard cell in the reference beam as in some other instruments.

Reflectance/Gel Scanning

Accessories include fixed angle and variable angle reflectance units and a gel scanning system for gels up to 100mm long.

Multi-User Convenience

Aquarius double beam instruments were designed for the greatest convenience in a laboratory where multi user operation is required.  All methods, stored scans, plots, curves and data remain in secure integral memory, tamper protected by passwords, readily accessible to all operators without the possibility of mislaid disks or plug in card modules.

Cells and Flowcells

As well as 10mm cells and flowcells, holders are available for rectangular and cylindrical cells up to 100mm pathlength. A microsampling system measures to 50ml or below.

Automatic Cell Changers

Automatic changers are available for 4, 6 or 8 cells, providing rapid sample changing or the facility for full programming.

Sipette Sampling

Samples down to 300 microlitres may be measured in a 10mm cell. Sampling is under control of the instrument's microprocessor system. Samples may be saved or pumped to waste.

Ultra-Micro Sampling

Valuable samples, such as biological samples prepared with costly reagents, may be measured using a special ultra-microcell and holder. With the system, samples of 50µl or even less may be accurately measured.  Third party nano cells may also be used.

Sample Temperature Control

Water and thermoelectric temperature control are available for single cells and automatic cell changers. Temperature is set from the control panel and displayed on the screen.

Dissolution Testing

Versions of the instruments fitted with a special cell compartment with piping port, are available for dissolution testing using Cecil TD software and a PC. Six and eight cell changers are available.

Batch Sampling

Automatic batch sampling for up to 100 samples is available. The pump fits within the sample compartment and volume etc is set from the control panel and displayed on screen. Batch number entry is provided.

Specular Reflectance

Special accessories enable specular reflectance measurements to be made. Both fixed angle and variable angle reflectance accessories are available. The latter is particularly useful for film thickness measurements.

Gel Scanning

A gel scanner enables gels up to 100mm long to be measured. An autoradiograph film holder is also available.

Micro - Sipette Sampling

Small samples or multiple samples are conveniently measured using the micro-sipette system, with very low cross contamination.  All measurements are made in the same cell, enhancing accuracy.  The pump, which is mounted in the sample compartment, operates under the control of the instrument's micro-processor system.
Assay Programmes

Reaction Kinetics

Both single cell and multi cell Kinetics are available with reaction plots to screen and printer. Regression analysis is applied to the user selected portion of reaction curves and results are reported in Units/Litre. Data may be reprocessed as required and curves stored. Sample temperature control is available for single cells and four and six cell changers.

Advanced Kinetics

An example of advanced kinetics is shown below where time based reaction data generated by the spectrophotometer, has been transferred into Grafit, fitted to a curve and transformed into a more suitable format for mathematical interpolation and calculation. In this particular example, the basic data plot can yield the limiting rate (Vmax), the Michaelis constant (Km) and the initial linear portion (Vmax/Km).

This has been transformed into the Eadie-Hofstee format and plotted to give better precision in the calculation of these important factors and constants.

Other transformations are available such as Lineweaver-Burk and the Hill plot for allosteric enzyme systems.

PC Data Manipulation

Using DataStream software, data may be transferred to a PC for use with spread sheets and other mathematical software packages.

More advanced reaction kinetics data manipulation may be readily performed with Grafit software. Linear, non-linear or polynomial curve fitting is easily accomplished together with non-linear regression analysis. A wide selection of standard equations are provided, together with an equation editor for customised equation fitting.

Data may also be transformed into other mathematical formats for plotting and calculation.

Band Quantification

Accurate quantitative assays using band area or height, corrected for background absorption, are exceptionally easy to carry out with the Series 7000 Aquarius instruments.

Analytical results are displayed on the screen with construction lines enabling the quality of the parameters to be assessed.

The procedure may be stored as a method with security code protection in the integral memory of the instrument.
   
Validation

Assessment of instrument performance is readily carried out using the validation software module built into all Aquarius instruments and released by E-SEF, 'encoded software enhancement facility'. Laboratories subject to regulatory control, or following G.L.P. are provided with a range of Pharmacopoeia (EP) tests as well as tests using filters traceable to national standards with certificates of calibration issued by Cecil Instruments. A selection of the tests are shown here.

Absorbance Accuracy

The Pharmacopoeia (EP) test for Absorbance accuracy, using a 60mg/L solution of Potassium Dichromate in a 10mm pathlength cuvette, is shown here.

Optical Bandwidth

The optical bandwidth of the instrument may be validated by measuring the half height width of the narrow deuterium emission line at 656.1nm. The measurement is carried out automatically.

Validation Using Filters

Absorbance accuracy is most conveniently measured using certified filter sets calibrated by Cecil Instruments against NPL certified standards. A validation printout for the procedure is shown here. Wavelength calibration is also carried out using certified filters.
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系統(tǒng)功能







雙光束光學(xué)系統(tǒng)

完全對稱的光學(xué)系統(tǒng)可以同時測量樣品和參比的最終準確度，精密度和基線平直度。長期穩(wěn)定性達到比單波束系統(tǒng)好十倍。




積分控制系統(tǒng)

所有的控制軟件就是儀器內(nèi)，防篡改，提供簡單的菜單驅(qū)動操作進行大規(guī)模的安全存儲掃描，繪圖，曲線等PC控制和數(shù)據(jù)傳輸?shù)絇C提供。




等于樣品和參考設(shè)備

相同大方樣品和參比采樣設(shè)施加寬的測量范圍，例如，為了使多細胞采樣在兩梁和容納笨重配件參考光束中。




方法操作和儲存

多達30套的儀器參數(shù)，包括濃度曲線可以存儲，安全代碼的保護。當(dāng)回憶說，儀器參數(shù)都自動設(shè)置，避免錯誤。




字母數(shù)字輸入

提供完整的字母數(shù)字項，這樣的結(jié)果可能會完全記錄的GLP 。公式輸入支持創(chuàng)建各種公式使用多個波長自動計算。




軟件增強通過E- SEF

E- SEF - 編碼軟件增強基金 - 允許使用通過電話，傳真或電子郵件提供的編碼數(shù)字被激活的軟件模塊。軟件要求可定制的，在任何時候，根據(jù)需要。




數(shù)據(jù)存儲和保護

數(shù)據(jù)，其中包括100光譜，濃度曲線，動力學(xué)和時間進程曲線可以存儲在安全的存儲，使用一個四位數(shù)的密碼安全密碼保護，防止篡改或刪除。




后處理

掃描，時間過程圖，并反應(yīng)曲線可以全部進行再加工，以在顯示屏幕上的任何所需的格式，然后繪制在積分或外部打印機。




性能驗證

軟件和認證標準，使波長準確度，吸光度精度，帶寬等進行驗證。一個定時及于報告包括儀器的序列號。
定量




強大的定量

許多定量方法可供選擇，包括帶面積計算與背景校正，差分和光譜，酒和彩色分析，幾個波長，蛋白質(zhì)和DNA檢測的數(shù)學(xué)計算的剝離。




曲線和擬合線

線性，二次或三次濃度曲線可裝配到高達30標準，使用或不使用截距在原點。標準可能會被刪除并替換和新的曲線擬合。




反應(yīng)動力學(xué)

單細胞動力學(xué)測量是可能的情節(jié)和畫面上顯示的數(shù)據(jù)。數(shù)據(jù)可能會被重新處理，回歸分析應(yīng)用和曲線存儲，安全代碼保護，防止篡改或刪除。




多細胞反應(yīng)動力學(xué)

多達四個或六個細胞與屏幕上顯示的所有反應(yīng)曲線自動測量。每條曲線可用于處理被選擇與回歸分析印刷的結(jié)果。




多波長測定

使用兩個或三個波長的光被提供的檢測。此酒顏色鑒定提供一種可使用多達十個不同的波長。還提供計算在多達四個波長。




DNA和蛋白質(zhì)測定

蛋白質(zhì)檢測可以使用華寶和基督教無論是2或3波長的方法進行。也編有技術(shù)和實驗分析程序被凱爾 - 洛瑞，布拉德福德，縮二脲和BCA方法。




帶面積和高度計算

與校正傾斜的背景吸收帶區(qū)域容易量化，并顯示所有的細節(jié)和建設(shè)。帶區(qū)域也可以被計算為微分光譜，以便難以定量。




波長程序

使用各種細胞變換器，測量可以由多達4或6或8個細胞在含有周期時間和由操作者輸入的周期數(shù)的單一波長。的細胞和波長程序可被組合。




細胞程序

測量可以被編程為10個不同的操作員所選擇的波長的周期時間和由操作者選定的周期數(shù)。波長和細胞程序可被組合。
掃描




雙光束波長掃描

罐高達4000nm/min呈現(xiàn)在屏幕上或印有注釋的波長和吸收所有的山峰。表中還提出，列出所有的波峰和波谷與價值觀。




滾動顯示

使用顯示滾動設(shè)施的提高有效屏幕尺寸，使光譜和數(shù)據(jù)高達550nm的寬來看待。




動態(tài)掃描召回

八強掃描通過輸入其掃描順序號自動保留和召回可能被再加工，重疊或轉(zhuǎn)移到受保護的內(nèi)存以備將來調(diào)用。




重疊光譜掃描

多達八個掃描，包括存儲的掃描，也可以重疊，帶或不帶偏移量為便于比較，或進一步的操作。




差譜

從存儲新的波長掃描或掃描可以被呈現(xiàn)在屏幕上，所產(chǎn)生的差示光譜。該掃描可以被歸一化使得頻譜剝露出的雜質(zhì)或添加劑。




導(dǎo)數(shù)光譜

微分光譜，上升到第四易于生產(chǎn)的，具有可選的平滑處理，無論是單獨或疊加在基本的，帶或不帶偏移量。縮放是自動與量化所有的波峰和波谷。




掃描存儲

高達100光譜可以存儲在10安全存儲銀行以備將來調(diào)用。每個光譜是自動分配的召回數(shù)，存儲時，也可以安全碼保護，以防止篡改或刪除。




編程掃描

自動掃描，單次或重復(fù)可以由一個單元程序的最多4個， 6個或8個細胞。掃描的編排順序也可以由一個單一的樣本。




時間課程地塊

更換樣品或流量可檢測并顯示通過做一個時間過程的情節(jié)。數(shù)據(jù)可能會重新處理和存儲，安全代碼保護自動分配的召回數(shù)量。
采樣




采樣靈活性

雙光束光采樣的設(shè)計允許在這兩個樣品光束和參考光束完全相似的空間和設(shè)備。因此，全尺寸的配件和多單元格引用的采樣，隨溫度的控制是可能的。操作不限于參考光束在單細胞水平在某些其他工具。




反射/凝膠掃描

附件包括固定角度和變角反射單元和凝膠可達100mm長的凝膠掃描系統(tǒng)。




多用戶便利

水瓶座雙光束儀器被設(shè)計為在需要的多用戶操作實驗室最大的便利。所有的方法，存儲掃描，圖形，曲線和數(shù)據(jù)留在安全的整體記憶，篡改受密碼保護，容易接觸到所有運營商沒有放錯盤的可能性或插入卡模塊。



 
細胞和測量管

以及10毫米細胞和測量管，支架可用于矩形和圓柱形電池可達100mm光程。一個微量進系統(tǒng)的措施至50ml以下。




自動換電池

自動換可用于4,6或8個細胞，提供快速樣品更換或設(shè)施進行全面編程。




Sipette采樣

樣品下降到300微升可以以10mm比色皿進行測定。抽樣是根據(jù)儀器的微處理器系統(tǒng)的控制權(quán)。樣品可以保存或抽浪費。




超微型采樣

有價值的樣品，如制備昂貴試劑的生物樣品，可以使用特殊的超微小區(qū)和保持器測定。用系統(tǒng)中， 50μl的或什至更少的樣品可以被精確地測量。第三方納米細胞也可使用。




樣品溫度控制

水和熱電溫度控制可用于單個細胞和細胞自動換。溫度從控制面板中設(shè)置和顯示在屏幕上。




溶出度測試

配有特殊的細胞室與配管口的工具版本，可使用塞西爾TD軟件和PC溶出度測試。六到八個細胞兌換服務(wù)。




批量采樣

自動批量采樣多達100個樣品是可用的。樣品室中的泵適合，體積等從控制面板中設(shè)置并在屏幕上顯示出來。提供批號條目。




鏡面反射

特殊配件使鏡面反射率測量作出。兩個固定角度和變角反射附件可供選擇。后者是用于薄膜厚度測量時特別有用。




凝膠掃描

凝膠掃描儀使凝膠可達100mm長的時間來衡量。放射自顯影膠片支架也可以。




微 - Sipette采樣

小樣品或多個樣品用微sipette系統(tǒng)可方便地測得，具有非常低的交叉污染。所有的測量都在相同的細胞制成，提高精度。泵，其被安裝在樣品室中，操作該儀器的微型處理器系統(tǒng)的控制下。
試驗計劃




反應(yīng)動力學(xué)

單細胞和多細胞動力學(xué)可與反應(yīng)陰謀屏幕和打印機。回歸分析應(yīng)用到反應(yīng)曲線和結(jié)果的用戶選擇的部分中報告單位/升。根據(jù)需要和曲線存儲的數(shù)據(jù)可以被重新處理。樣品溫度控制可用于單細胞和四和六單元換。




高級動力學(xué)

先進的動力學(xué)的例子所示，其中由分光光度計產(chǎn)生基于時間的反應(yīng)數(shù)據(jù)，已轉(zhuǎn)移到GRAFIT ，裝到一個曲線并轉(zhuǎn)化為一個更合適的格式為數(shù)字內(nèi)插和計算。在這個特定的例子中，基本的數(shù)據(jù)圖可以得到限流速率（Vmax ） ，米氏常數(shù)（公里），并在初始線性部分（速度/公里） 。




這已轉(zhuǎn)化為伊迪 - Hofstee格式和密謀提供更好的精度在這些重要因素和常數(shù)的計算。




其他轉(zhuǎn)換可如雙倒數(shù)和積山的別構(gòu)酶系統(tǒng)。




電腦數(shù)據(jù)操作

使用的DataStream軟件，數(shù)據(jù)可傳輸?shù)诫娔X與電子表格和其他數(shù)學(xué)軟件包的使用。




更高級的反應(yīng)動力學(xué)數(shù)據(jù)操作可以很容易地進行與GRAFIT軟件。線性，非線性或多項式曲線擬合很容易用非線性回歸分析來實現(xiàn)在一起。提供了多種選擇的標準方程，連同一個公式編輯器定制方程擬合。




數(shù)據(jù)也可以轉(zhuǎn)換成其他的數(shù)學(xué)格式繪制和計算。







帶定量

使用帶面積或高度準確的定量分析，為背景吸收校正，是非常容易執(zhí)行與7000系列水瓶座儀器。




分析結(jié)果顯示在屏幕上，建筑線條使被評估參數(shù)的質(zhì)量。




該程序可以被存儲為與安全碼保護儀器的積分存儲器的方法。
    
驗證




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儀器性能評估是很容易進行了使用內(nèi)置到所有水瓶座儀器和電子海基會， “編碼軟件增強工具”發(fā)布了驗證軟件模塊。實驗室受監(jiān)管控制，或以下的GLP提供了一系列藥典（ EP）的測試，以及測試使用過濾器可溯源至國家標準與塞西爾儀器發(fā)出的校準證書。一個選擇的測試都在這里顯示。




吸光度準確度

藥典（ EP）測試吸光度準確度，用重鉻酸鉀的60毫克/ L的溶液采用10mm光程的比色皿，在這里顯示。







光學(xué)帶寬

儀器的光學(xué)帶寬可以通過測量窄氘發(fā)射線的半高寬在656.1nm進行驗證。測量自動進行。




驗證使用過濾器

吸光度準確度使用由塞西爾儀器對不良貸款認證標準校準認證的過濾器設(shè)置最方便的測量。一個驗證打印輸出的程序如下所示。波長校準也進行了使用認證的過濾器。

 技術(shù)規(guī)格：

Optical System	Fully symmetrical double beam optical system. All mirrors coated for long life
Monochromator	Modified Czerny-Turner design using 1200 L/mm holographic diffraction grating
Detectors	Two silicon diode detectors
Self Test and Calibration	Automatic at switch on
Wavelength Range and Accuracy	190 - 1100nm; 0.5nm  (For CE 7200, 190 - 900nm; 0.5nm)
Wavelength Reproducibility	0.1nm
Wavelength Readout	Digital display on screen
Wavelength Selection	By GOTO Key and keypad entry
Optical Bandwidth	For CE 7200 and CE 7400 1.8 nm.    For CE 7500 user selectable, 0.5, 1, 1.8 and 4 nm.  For CE 7400S 1 nm
Stray Light	Less than 0.02% at 220nm, NaI; and 0.02% at 340nm, NaNO2
Display Screen	Large backlit LCD displays spectra, menus, data, operator prompts and instrument status.
Derivative Spectra	1st to 4th derivatives - autoranged or operator defined.
Autozero	Automatic by press button
Photometric Readout	Digital display of A, %T and C on screen
Photometric Accuracy	±.004A at 1A
Photometric Noise	Less than ±0.0001A at 550nm
Baseline	Stored in safe memory
Baseline Flatness	±0.001A (300nm/min, 200-900nm)
Baseline Stability	Better than ±0.0001A/hour
Computer Interface	Bi-directional RS 232  and USB ports
Scan Speeds	1-4000nm/min
Wavelength Scale Expansions	1-100nm/cm
Method Storage	30 methods may be stored and retrieved
Method Protection	Each protected by 4 digit password
Reaction Kinetics	With plots, reprocessing of data as required, for single cell or up to 6 cells
Overlaid Scans	Derivatives and scans superimposed with or without offset. Up to 8 scans may be overlaid.
Cell Programming	Up to 4, 6 or 8 cells
Programme Cycles	From 1 to 9999
Programme Delay (cycle time)	From 0 seconds to 4 days (1 sec resolution)
Wavelength Programming	Up to 10 wavelengths. May be combined with a cell programme
Storage of Spectra	100 spectra may be stored security code protected
Concentration Curve Fitting	Linear, quadratic or cubic with editing of standards. Up to 30 curves may be stored and protected
Batch Sampling	For up to 100 samples under internal control with screen display
Date and Time Information	Printed from real time clock
Power Requirements	110-250V, 50/60Hz, 200 watts.
Dimensions	635 x 410 x 210mm
Weight	33kg

CE 7200 Spectrophotometer

1.8 nm Optical bandpass. Wavelength range 190-900nm.

CE 7260 Dissolution Spectrophotometer

Suitable for tablet dissolution monitoring using a choice of dissolution software and an automatic six or eight cell changer.

CE 7400 Spectrophotometer

1.8 nm Optical bandpass. Wavelength range 190-1,100nm.

CE 7460 Dissolution Spectrophotometer

Suitable for tablet dissolution monitoring using a choice of dissolution software and an automatic six or eight cell changer.

CE 7400S Spectrophotometer

1 nm Optical bandpass. Wavelength range 190-1,100nm.

CE 7460S Dissolution Spectrophotometer

Suitable for tablet dissolution monitoring using a choice of dissolution software and an automatic six or eight cell changer.

CE 7500 Spectrophotometer

User selectable 0.5, 1, 1.8 and 4 nm Optical bandpasses. Wavelength range 190-1,100nm.

CE 7560 Dissolution Spectrophotometer

Suitable for tablet dissolution monitoring using a choice of dissolution software and an automatic six or eight cell changer.